14-3-3z(Phospho-Ser58) Antibody

$454.00

Cat. No.: 39013 Categories: ,

Description

Aliases

1433Z; 143Z; FAS; Factor activating exoenzyme S; KCIP-1

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: P63104
NCBI Protein: NP_001129171.

Immunogen

Peptide sequence around phosphorylation site of serine 58 (R-S-S(p)-W-R) derived from Human 14-3-3 zeta.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IHC IF Recommended dilution: Predicted MW: 28kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Background / Function

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5′ UTR but that encode the same protein have been identified for this gene.Gu YM, et al. (2006) FEBS Lett ; 580(1): 305-310Powell DW, et al. (2003) Mol Cell Biol; 23(15): 5376-5387Mackintosh C. (2004) Biochem J; 381(Pt 2): 329-342.

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Ser58

Additional information

Size

100μl

Certificate of Analysis