BP-1; 4EBP1; 4E-BP1; PHAS-I;
Swiss-Prot: Q13541; NCBI Gene ID: 1978; NCBI mRNA:
Peptide sequence around phosphorylation site of threonine 36(S-T-T(p)-P-G) derived from Human 4E-BP1.
Human Mouse Rat
WB IHC Recommended dilution: Predicted MW: 18kd, Western blotting : 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.