Description
Aliases
BP-1; 4EBP1; 4E-BP1; PHAS-I;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q13541; NCBI Gene ID: 1978; NCBI mRNA:
Immunogen
Peptide sequence around phosphorylation site of threonine 36(S-T-T(p)-P-G) derived from Human 4E-BP1.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC Recommended dilution: Predicted MW: 18kd, Western blotting : 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Thr36
Additional information
| Size | 100?l |
|---|
