4E-BP1(Phospho-Thr45) Antibody

$476.70

Cat. No.: 38806 Categories: ,

Description

Aliases

EIF4EBP1; PHAS-1;

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: Q13541
NCBI Protein: NP_004086.1

Immunogen

Peptide sequence around phosphorylation site of threonine 45 (S-T-T(p)-P-G) derived from Human 4E-BP1.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IHC Recommended dilution: Predicted MW: 18kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Background / Function

4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5′ end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. Gingras AC, et al. (1998) Genes Dev 12(4): 502-513. Brugarolas J, et al. (2004) Genes Dev 18(23): 2893-2904. Kumar V, et al. (2000) EMBO J 19(5): 1087-1097. Moody CA, et al. (2005) J Virol 79(9): 5499-5506. Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95(4): 1432-1437.

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Thr45

Additional information

Size

100?l

Certificate of Analysis