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  • Anti-DYKDDDDK (FLAG) Tag, Clonility FG4R - 50 µg

Anti-DYKDDDDK (FLAG) Tag, Clonility FG4R - 50 µg

Product Code:10009
Availability:In Stock
  • $116.00


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Source protein:           

Protein A affinity chromatography purified protein from mouse ascites fluid.


Clonality and Host Species:    

FG4R, Mouse


Immunogen and Isotype:        

DYKDDDDK (FLAG) synthetic peptide conjugated to KLH, IgG1


Specificity and reactivity:       

Detects the N-terminal or C-terminal FLAG -tagged fusion proteins.

 

Application:                              

Western blot, Dot blot, ELISA, IHC/IS, Western blot (with ECL) 1-5 ng of DYKDDDDK -tagged fusion protein.


Available form:                        

Lyophilized 1mg/mL (lyophilized in 10mM PBS, pH 7.2 with 1% BSA and 0.05% Sodium azide; NaN3)

                                     
Working procedure:                

Western blot (with ECL) 1:1000-3,000 dilution incubate for 1 hour at room temperature. For the best results, determine the optimal working antibody dilution for your protein of interest.


Storage:                                

Supplied lyophilized antibodies can be stored at -200C for 3 years.

Reconstitute the lyophilized antibody with distilled water to 1mg/ml or a desired concentration before use. 

Reconstituted liquid can be stored at -20oC for 2 years. Centrifuge after first thaw to maximum product recovery. Aliquot into smaller volume to avoid repeated freeze-thaw cycles.  Store aliquots at 40C for several days to 2 weeks.