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  • Anti-His Tag, Clonility HIS.H8 - 50 µg

Anti-His Tag, Clonility HIS.H8 - 50 µg

Product Code:10004
Availability:In Stock
  • $116.00


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Source protein:           

Protein A affinity chromatography purified protein from mouse ascites fluid.


Clonality and Host Species:    

HIS. H8, Mouse


Immunogen and Isotype:        

HHHHHH (6x His) synthetic peptide conjugated to KLH, IgG2b


Specificity and reactivity:       

Detects the epitope of 6x His-Tags encoded by many commercially available vectors, regards less of the tag’s location in the fusion protein sequences (reacts with N-terminal, C-terminal or internal 6X His-tags).

 

Application:                              

Western blot, Dot blot, ELISA, IHC/IS, Western blot (with ECL) 1-2 ng of His-tagged fusion protein.


Available form:                        

Lyophilized 1mg/mL (lyophilized in 10mM PBS, pH 7.2 with 1% BSA and 0.05% Sodium azide; NaN3)

                                        
Working procedure:                

Western blot (with ECL) 1:1000-3,000 dilution incubates for 1 hour at room temperature. For the best results, determine the optimal working antibody dilution for your protein of interest.

Storage:                                

Supplied lyophilized antibodies can be stored at 200C for 3 years.

Reconstitute the lyophilized antibody with distilled water to 1mg/ml or a desired concentration before use. 

Reconstituted liquid can be stored at -20oC for 2 years. Centrifuge after first thaw to maximum product recovery. Aliquot into smaller volume to avoid repeated freeze-thaw cycles.  Store aliquots at 40C for several days to 2 weeks.