Protein A affinity
chromatography purified protein from mouse ascites fluid.
Clonality and Host Species:
HIS. H8, Mouse
Immunogen and Isotype:
HHHHHH (6x His) synthetic
peptide conjugated to KLH, IgG2b
Specificity and reactivity:
Detects the epitope of
6x His-Tags encoded by many commercially available vectors, regards less of the
tag’s location in the fusion protein sequences (reacts with N-terminal,
C-terminal or internal 6X His-tags).
Western blot, Dot blot,
ELISA, IHC/IS, Western blot (with ECL) 1-2 ng of His-tagged fusion protein.
Lyophilized 1mg/mL (lyophilized
in 10mM PBS, pH 7.2 with 1% BSA and 0.05% Sodium azide; NaN3)
Western blot (with ECL) 1:1000-3,000
dilution incubates for 1 hour at room temperature. For the best results,
determine the optimal working antibody dilution for your protein of interest.
antibodies can be stored at 200C for 3 years.
lyophilized antibody with distilled water to 1mg/ml or a desired concentration
Reconstituted liquid can
be stored at -20oC for 2 years. Centrifuge after first thaw to
maximum product recovery. Aliquot into smaller volume to avoid repeated
freeze-thaw cycles. Store aliquots at 40C
for several days to 2 weeks.