Description
Aliases
NACP; SYN; SYUA; alpha-synuclein;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P37840
NCBI Protein: NP_000336.1
Immunogen
Peptide sequence around phosphorylation site of tyrosine 125 (E-A-Y(p)-E-M) derived from Human a-Synuclein.
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
WB IF Recommended dilution: Predicted MW: 18kd, Western blotting: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background / Function
SncA is a member of the synuclein family of structurally related proteins that are prominently expressed in the central nervous system, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and SncA and Snc-Beta inhibit phospholipase D2 selectively. SncA may serve to integrate presynaptic signaling and membrane trafficking. Aggregated SncA proteins form brain lesions that are hallmarks of neurodegenerative synucleinopathies. Defects in SncA play a role in the pathogenesis of Parkinson disease. SncA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer disease. SncA shares 95% sequence homology with rat SncA. Rat SncA is specifically expressed in brain and is associated with synaptosomal membranes in neurons Takahashi T, et al. J Biol Chem 2003 Oct 24; 278(43): 42225-33 Ahn BH, et al. J Biol Chem 2002 Apr 05; 277(14): 12334-42 Negro A, et al. FASEB J 2002 Feb; 16(2): 210-2 Goldberg, et al. Nat. Cell Biol. 2000; 2, 115-119.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Tyr125
Additional information
| Size | 100?l |
|---|
