Acetylcholinesterase (AChE) is known to be one of the most important enzymes involved in neural responsiveness and overall functionality. Its main responsibility is breaking down acetylcholine (ACh), as a result forming acetic acid and choline. Used to stop synaptic transmission, AChE is often located near the central nervous system’s cholinergic synapses, as well as neuromuscular junctions.
Cepham Life Sciences provides a kit with sensitive detection of AChE activity. Cell extracts, blood samples, and other solutions can be analyzed for AChE activity, using DTNB to quantify thiocholine, a product of acetylthiocholine hydrolysis by AChE. To directly measure the level of AChE activity, the production of thiocholine is determined by observing the DTNB absorption intensity.
100 Assays, 200 Assays