AFX(Phospho-Ser197) Antibody

$454.00

Cat. No.: 39004 Categories: ,

Description

Aliases

AFX; FOXO4; AFX1; Afxh;

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: P98177
NCBI Protein: NP_001164402.

Immunogen

Peptide sequence around phosphorylation site of serine 197 (A-A-S(p)-M-D) derived from Human AFX.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IHC IF Recommended dilution: Predicted MW: 65kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Background / Function

Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Di Maira G, et al. (2005)Cell Death Differ; 12(6): 668-77.Essers MA, et al. EMBO J 2004 Nov. 11.Brownawell AM, (2001) Mol Cell Biol; 21(10): 3534-46.Kops GJ, et al. (1999) Nature; 398(6728): 630-4.

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Ser197

Additional information

Size

100?l

Certificate of Analysis