PKB beta; Protein kinase B; RAC-PK-beta
NCBI Protein: NP _001617.1
Peptide sequence around phosphorylation site of serine 474 (Q-F-S(p)-Y-S) derived from Human Akt2.
Human Mouse Rat
WB IHC Recommended dilution: Predicted MW: 60kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
General protein kinase capable of phosphorylating several known proteins. Sun M, et al. (2001) Cancer Res; 61(16): 5985-91. Yuan ZQ, et al. (2000) Oncogene; 19(19): 2324-30. Meier R, et al. (1997) J Biol Chem; 272(48): 30491-7.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.