AMPKa1(Phospho-Ser487)Antibody

$454.00

Cat. No.: 39093 Categories: ,

Description

Aliases

AAPK1; AMPK alpha-1 chain; AMPK-alpha1; HMG-CoA redustase kinase; PRKAA1

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: Q13131/P54646
NCBI Protein: NP_006

Immunogen

Peptide sequence around phosphorylation site of serine 487 (S-G- SP-V-S) derived from Human AMPKa1.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IHC IF Recommended dilution: Predicted MW: 63kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Background / Function

Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5′-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. Kim JE, et al. (2005) J Proteome Res. 4(4): 1339-1346. Woods A, et al. (2003) J Biol Chem. 278(31): 28434-28442.

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Ser487

Additional information

Size

100?l

Certificate of Analysis