Anti-DYKDDDDK (FLAG) Tag, Clonility FG4R

$125.00$226.00

Cat. No.: 10009 Categories: ,

Description

Source protein:           

Protein A affinity
chromatography purified protein from mouse ascites fluid.



Clonality and Host Species:    

FG4R, Mouse



Immunogen and Isotype:        

DYKDDDDK (FLAG) synthetic
peptide conjugated to KLH, IgG1



Specificity and reactivity:       

Detects the N-terminal
or C-terminal FLAG -tagged fusion
proteins.

 

Application:                              

Western blot, Dot blot,
ELISA, IHC/IS, Western blot (with ECL) 1-5 ng of DYKDDDDK -tagged fusion protein.



Available form:                        

Lyophilized 1mg/mL (lyophilized
in 10mM PBS, pH 7.2 with 1% BSA and
0.05%
Sodium azide; NaN3)

                                     
Working procedure:                

Western blot (with ECL)
1:1000-3,000 dilution incubate for 1 hour at room temperature. For the best
results, determine the optimal working antibody dilution for your protein of
interest.



Storage:                                

Supplied lyophilized
antibodies can be stored at -200C for 3 years.

Reconstitute the
lyophilized antibody with distilled water to 1mg/ml or a desired concentration
before use. 

Reconstituted liquid can be
stored at -20oC for 2 years. Centrifuge after first thaw to maximum
product recovery. Aliquot into smaller volume to avoid repeated freeze-thaw cycles.  Store aliquots at 40C for several
days to 2 weeks.

Additional information

Size

50 µg, 100 µg

Certificate of Analysis