Description
Aliases
ARR1; arrestin 2; arrestin beta 1; beta-arrestin-1;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot#: P49407;
NCBI Gene#: 408;
NC
Immunogen
Peptide sequence around phosphorylation site of Serine 412 (T-G-S(p)-P-Q) derived from Human Arrestin 1.
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
WB IHC Recommended dilution: Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
Functions in regulating agonist-mediated G-protein coupled receptor (GPCR) signaling by mediating both receptor desensitization and resensitization processes. During homologous desensitization, beta-arrestins bind to the GPRK-phosphorylated receptor and sterically preclude its coupling to the cognate G-protein; the binding appears to require additional receptor determinants exposed only in the active receptor conformation. Greg Buchanan. AACR Meeting Abstracts, Apr 2006; 2006: 430. D. Alex Groarke. Mol. Pharmacol., Feb 2001; 59: 375. Karen McConalogue. Mol. Biol. Cell, Aug 1998; 9: 2305. Maria Stella Lombardi. Stroke, Apr 2004; 35: 981 – 986.
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser412
Additional information
| Size | 100?l |
|---|
