ASK-1; M3K5; MAP3K5; MAPK/ERK kinase kinase 5; MAPKKK5
NCBI Protein: NP_005914.1
Peptide sequence around phosphorylation site of serine 83 (G-S-S(p)-V-G) derived from Human ASK1.
WB IHC Recommended dilution: Predicted MW: 155kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death. Mabuchi S, et al. (2004) Endocrinology. 145(1): 49-58. Yuan ZQ, et al. (2003) J Biol Chem. 278(26): 23432-23440. Kim AH, et al. (2001) Mol Cell Biol. 21(3): 893-901.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.