Description
Aliases
ASK-1; M3K5; MAP3K5; MAPK/ERK kinase kinase 5; MAPKKK5
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q99683
NCBI Protein: NP_005914.1
Immunogen
Peptide sequence around phosphorylation site of serine 966 (S-I-S(p)-L-P) derived from Human ASK1.
Raised In
Rabbit
Species Reactivity
Human Mouse Monkey
Tested Applications
WB IHC Recommended dilution: Predicted MW: 155kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death. Zhang W, et al. (2005) J Biol Chem. 280(19): 19036-19044. Fujii K, et al. (2004) Oncogene. 23(29):5099-5104. Goldman EH, et al. (2004) J Biol Chem 2004 Mar 12; 279(11): 10442-10449. Zhang L, et al. (1999) Proc Natl Acad Sci U S A. 96(15): 8511-8515.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser966
Additional information
| Size | 100?l |
|---|
