NCBI Protein: NP_001871.2
Peptide sequence around phosphorylation site of threonine 71 or 53 (T-P-T(p)-P-T) derived from Human ATF2.
Human Mouse Rat
WB IHC Recommended dilution: Predicted MW: 65-75 kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5′-GTGACGT[AC][AG]-3′), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex. Sevilla A, et al. (2004) J Biol Chem. 279(26):27458-27465. Waetzig G H, et al. (2002) J Immunol. 168(10): 5342-5351. Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089. Gupta S, et al. (1995) Science. 267: 389-393. Van Dam H, et al. (1995) EMBO J. 14(8): 1798-1811.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Phospho-Thr71 or 53