Ataxia telangiectasia mutated homolog; Ataxia telangiectasia mutated; kinase ATM
NCBI Protein: NP_000042.3
Peptide sequence around phosphorylation site of serine 1981 (E-G-S(p)-Q-S) derived from Human ATM.
WB IHC Recommended dilution: Predicted MW: 350kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
ATM encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene. Gupta A. et al. (2005) Mol Cell Biol. 25(12): 5292-5305. Bernstein JL. et al. (2002) Breast Cancer Res. 4(6): 249-252. Silverman J. et al. (2004) Genes Dev. 18(17): 2108-2119. Nakada D. et al. (2003) Nucleic Acids Res. 31(6): 1715-1724.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.