ATM(Phospho-Ser1981) Antibody

$476.70

Cat. No.: 38772 Categories: ,

Description

Aliases

Ataxia telangiectasia mutated homolog; Ataxia telangiectasia mutated; kinase ATM

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: Q13315
NCBI Protein: NP_000042.3

Immunogen

Peptide sequence around phosphorylation site of serine 1981 (E-G-S(p)-Q-S) derived from Human ATM.

Raised In

Rabbit

Species Reactivity

Human Mouse

Tested Applications

WB IHC Recommended dilution: Predicted MW: 350kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Background / Function

ATM encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene. Gupta A. et al. (2005) Mol Cell Biol. 25(12): 5292-5305. Bernstein JL. et al. (2002) Breast Cancer Res. 4(6): 249-252. Silverman J. et al. (2004) Genes Dev. 18(17): 2108-2119. Nakada D. et al. (2003) Nucleic Acids Res. 31(6): 1715-1724.

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Ser1981

Additional information

Size

100?l

Certificate of Analysis