ATXN3 Antibody

$210.00$377.00

Cat. No.: 40096 Categories: ,

Description

Aliases

Ataxin-3, Machado-Joseph disease protein 1, Spinocerebellar ataxia type 3 protein, ATXN3, ATX3, MJD, MJD1, SCA3

Antibody Type

Polyclonal Antibody

Species

Human

Uniprot ID

P54252

Immunogen

Recombinant human Ataxin-3 protein (1-288AA)

Raised In

Rabbit

Species Reactivity

Human,Mouse

Tested Applications

ELISA, WB, IHC;Recommended dilution:WB:1:500-2000, IHC:1:20-1:200

Background / Function

Deubiquitinating enzyme involved in protein homeostasis maintenance, transcription, cytoskeleton regulation, myogenesis and degradation of misfolded chaperone substrates. Binds long polyubiquitin chains and trims them, while it has weak or no activity against chains of 4 or less ubiquitins. Involved in degradation of misfolded chaperone substrates via its interaction with STUB1/CHIP: recruited to monoubiquitinated STUB1/CHIP, and restricts the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. In response to misfolded substrate ubiquitination, mediates deubiquitination of monoubiquitinated STUB1/CHIP. Interacts with key regulators of transcription and represses transcription: acts as a histone-binding protein that regulates transcription.

Isotype

IgG

Conjugate

Unconjugated

Storage Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Caprylic Acid Ammonium Sulfate Precipitation purified

Literature

[1]”Deubiquitinating function of ataxin-3: insights from the solution structure of the Josephin domain.”Mao Y., Senic-Matuglia F., Di Fiore P.P., Polo S., Hodsdon M.E., De Camilli P.Proc. Natl. Acad. Sci. U.S.A. 102:12700-12705(2005). [2]”The solution structure of the Josephin domain of ataxin-3: structural determinants for molecular recognition.”Nicastro G., Menon R.P., Masino L., Knowles P.P., McDonald N.Q., Pastore A.Proc. Natl. Acad. Sci. U.S.A. 102:10493-10498(2005). [3]”Structural modeling of ataxin-3 reveals distant homology to adaptins.” Albrecht M., Hoffmann D., Evert B.O., Schmitt I., Wuellner U., Lengauer T. Proteins 50:355-370(2003).

Additional information

Size

50μg, 100μg

Certificate of Analysis