Aurora A(phospho-Thr288) Antibody

$476.70

Cat. No.: 38842 Categories: ,

Description

Aliases

AIK; ARK1; AURA; BTAK; STK6

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: O14965
NCBI Protein: NP_003591.2

Immunogen

Peptide sequence around phosphorylation site of threonine 288 (R-T-T(p)-L-M) derived from Human Aurora A.

Raised In

Rabbit

Species Reactivity

Human Mouse

Tested Applications

WB IHC Recommended dilution: Predicted MW: 48kd, Immunohistochemistry: 1:50~1:100

Background / Function

Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity By similarity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Bischoff, J.R. et al. (1998) EMBO J 17, 3052-65. Hauf, S. et al. (2003) J Cell Biol 161, 281-94. Walter, A.O. et al. (2000) Oncogene 19, 4906-16. Zhou, H. et al. (1998) Nat Genet 20, 189-93.

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Thr288

Additional information

Size

100?l

Certificate of Analysis