Description
Aliases
AIK; ARK1; AURA; BTAK; STK6
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: O14965
NCBI Protein: NP_003591.2
Immunogen
Peptide sequence around phosphorylation site of threonine 288 (R-T-T(p)-L-M) derived from Human Aurora A.
Raised In
Rabbit
Species Reactivity
Human Mouse
Tested Applications
WB IHC Recommended dilution: Predicted MW: 48kd, Immunohistochemistry: 1:50~1:100
Background / Function
Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity By similarity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Bischoff, J.R. et al. (1998) EMBO J 17, 3052-65. Hauf, S. et al. (2003) J Cell Biol 161, 281-94. Walter, A.O. et al. (2000) Oncogene 19, 4906-16. Zhou, H. et al. (1998) Nat Genet 20, 189-93.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Thr288
Additional information
Size | 100?l |
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