Description
Aliases
Bbc2
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q61337
NCBI Protein: NP_031548.1
Immunogen
Peptide sequence around phosphorylation site of serine 112 (H-S-S(p)-Y-P) derived from Mouse BAD.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC Recommended dilution: Predicted MW: 23kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. Zhang B, et al. (2004). Mol Cell Biol.24 (14): 6205-6214. Rice PL, et al. (2003). Cancer Res.63 (3): 616-620. Wang XQ, et al. (2001). J Biol Chem.276 (48): 44504-44511.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser112
Additional information
| Size | 100?l |
|---|
