BLNK (Phospho-Tyr84) Antibody

$476.70

Cat. No.: 38953 Categories: ,

Description

Aliases

B-cell linker protein; LY57; SLP-65; SLP65

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot#:Q8WV28;
NCBI Gene#:29760

Immunogen

Peptide sequence around phosphorylation site of tyrosine 84 (E-M-Y(p)-V-M) derived from Human BLNK.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IHC Recommended dilution: Western blotting: 1:500~1:3000, Immunohistochemistry: 1:50~1:100,

Background / Function

Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.

Conjugate

Unconjugated

Storage Buffer

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Tyr84

Additional information

Size

100?l

Certificate of Analysis