Description
Aliases
Signal transduction protein CBL; Proto-oncogene c-CBL; Casitas B-lineage lymphoma proto-oncogene; RING finger protein 55;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P22681
NCBI Protein: NP_005179.2
Immunogen
Peptide sequence around phosphorylation site of tyrosine 770 (T-E-Y(p)-M-T) derived from Human c-Cbl.
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
WB IHC IF Recommended dilution: Predicted MW: 120kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.Blake, T.J. et al. (1991) Oncogene 6, 653-657.Thien, C.B. and Langdon, W.Y. (1998) Immunol. Cell Biol. 76, 473-482.Kamei, T. et al. (2000) Int. J. Oncol. 17, 335-339.Hunter, C. et al. (1999) J. Biol. Chem. 274, 2097-2106.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Tyr700
Additional information
| Size | 100?l |
|---|
