KIT; CD117; SCFR; PBT;
NCBI Protein: NP_000213.1
Peptide sequence around phosphorylation site of tyrosine 936 (H-I-Y(p)-S-N) derived from Human c-Kit.
IHC WB Recommended dilution: Predicted MW: 120 145kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,
Background / Function
This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K)Martin, F.H. et al. (1990) Cell 63, 203-11.Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62.Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25.Nocka, K. et al. (1990) EMBO J 9, 1805-13.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.