Aliases
CAMKA
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q9UQM7
NCBI Protein: NP_057065.2
Immunogen
Peptide sequence around phosphorylation site of threonine 286 (Q-E-T(p)-V-D) derived from Human CaMKII.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB Recommended dilution: Predicted MW: 50kd, Western blotting: 1:500~1:1000
Background / Function
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity Pak JH, et al. Proc Natl Acad Sci U S A. 2000 Oct 10; 97(21): 11232-11237 Hudmon A, et al. J Cell Biol. Author manuscript; available in PMC 2006 May 7 Miller P, et al. PLoS Biol. 2005 Apr; 3(4): e107 Runyan JD, et al. Learn Mem. 2005 Mar; 12(2): 103-110.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Thr286
