Caspase-3, 7 Assay Kit, Fluorometric with Ac-DEVD-AFC substrate

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Cat. No.: 10073 Categories: ,

Description

Caspase-3, 7 Assay Kit, Fluorometric with Ac-DEVD-AFC substrate

 

General description

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The CPP32/Caspase-3 Fluorometric Protease Assay Kit provides a simple and convenient means for assaying the DEVD-dependent caspase activity. The assay is based on detection of cleavage of substrate DEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). DEVD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by CPP32 or related caspases, free AFC emits a yellow-green fluorescence (lmax = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-3/CPP32 activity.

 

Detection Method:         Fluorescence (400 nm excitation filter and 505 nm emission filter)

Species Reactivity:        Mammalian

Applications:                  Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis

Storage temp.:               -20°C

 

Features & Benefits:

  • Simple one-step procedure that takes ~1-2 hours
  • Fast and convenient
  • Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-3/CPP32 activity

 

Kit Components:

  • Cell Lysis Buffer
  • 2X Reaction Buffer
  • DEVD-AFC (1 mM)
  • DTT (1 M)

Additional information

Size

50 Assays, 100 Assays, 200 Assays