CDC25B (Phospho-Ser323) Antibody

$454.00

Cat. No.: 38679 Categories: ,

Description

Aliases

CDC25HU2; CDC25M2; Dual specificity phosphatase Cdc25B; EC 3.1.3.48; M-phase inducer phosphatase 2; MPIP2

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot#:P30305;
NCBI Gene#:994

Immunogen

Peptide sequence around phosphorylation site of serine 323 (S-P-S(p)-M-P) derived from Human CDC25B.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB Recommended dilution: Western blotting: 1:500~1:3000,

Background / Function

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

Conjugate

Unconjugated

Storage Buffer

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Ser323

Additional information

Size

100?l

Certificate of Analysis