CDC25HU2; CDC25M2; Dual specificity phosphatase Cdc25B; EC 18.104.22.168; M-phase inducer phosphatase 2; MPIP2
Peptide sequence around phosphorylation site of serine 323 (S-P-S(p)-M-P) derived from Human CDC25B.
Human Mouse Rat
WB Recommended dilution: Western blotting: 1:500~1:3000,
Background / Function
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.