CDC28; CDC2A; CDK1; Cyclin-dependent kinase 1;
NCBI Protein: NP_001163877.
Peptide sequence around phosphorylation site of tyrosine 15 (G-T-Y(p)-G-V) derived from Human CDC2.
Human Mouse Rat
WB Recommended dilution: Predicted MW: 34kd, Western blotting: 1:500~1:1000
Background / Function
Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Y Gu, et al. (1992) EMBO J. 11(11): 3995
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.