Chk1(Phospho-Ser280) Antibody

$454.00

Cat. No.: 38779 Categories: ,

Description

Aliases

CHEK1; Chk1;

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: O14757
NCBI Protein: NP_001107593.

Immunogen

Peptide sequence around phosphorylation site of serine 280 (V-T-S(p)-G-G) derived from Human Chk1.

Raised In

Rabbit

Species Reactivity

Human

Tested Applications

WB Recommended dilution: Predicted MW: 56kd, Western blotting: 1:500~1:1000

Background / Function

Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at ‘Ser-178’ and ‘Thr-507’ and phosphorylation of CDC25C at ‘Ser-216’ creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at ‘Ser-76’, ‘Ser-124’, ‘Ser-178’, ‘Ser-279’ and ‘Ser-293’ promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at ‘Thr-309’, which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at ‘Ser-743’, which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation. Conn CW, et al. (2004)Dev Cell; 7(2): 275-81 King FW, et al. (2004)Cell Cycle; 3(5): 634-7 Shtivelman E, et al. (2002) Curr Biol; 12(11): 919-24

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Ser280

Additional information

Size

100?l

Certificate of Analysis