Description
Aliases
CFL; CFL1;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P23528
NCBI Protein: NP_005498.1
Immunogen
Peptide sequence around phosphorylation site of serine 3 (M-A-S(p)-G-V) derived from Human cofilin.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC IF Recommended dilution: Predicted MW: 19kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.Kobayashi M, et al. (2006) EMBO J 25(4): 713-26.Wang Y, et al. (2005) Biol Chem 280(13): 12683-9.Smith-Beckerman DM, et al. (2005) Mol Cell Proteomics: 156-68.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser3
Additional information
| Size | 100μl |
|---|
