Description
Aliases
CX43; CXA1; CXN-43; GJA1;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P17302
NCBI Protein: NP_000156.1
Immunogen
Peptide sequence around phosphorylation site of serine 368(R-A-S(p)-S-R) derived from Human Connexin 43.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC Recommended dilution: Predicted MW: 43kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Joell L. Solan1, et al. (2003) Cell Science 116: 2203-2211 Satoshi Matsushita, et al. (2006) Histochemistry and Cytochemistry 54 (3): 343-353, Xiaoyong Bao, et al. (2004) Cell Physiol 286: C647-C654 W. E. I. Li, et al.(1998) European Journal of Neuroscience 10: 2444
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser368
Additional information
| Size | 100?l |
|---|
