Amplaxin; CTTN; EMS1; SRC8;
NCBI Protein: NP_005222.2
Peptide sequence around phosphorylation site of tyrosine 466 (P-V-Y(p)-E-T) derived from Human CORTACTIN.
WB Recommended dilution: Predicted MW: 85kd, Western blotting: 1:500~1:1000
Background / Function
Cortactin is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene. Luo C, et al. (2006) J Biol Chem ; 281(40):30081-30093 Head JA, et al. (2003) Mol Biol Cell ;14(8):3216-3229 Li Y, et al. (2000) J Biol Chem ; 275(47): 37187-37193 Kinley AW, et al. (2003) Curr Biol ; 13(5): 384-393
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.