Description
Aliases
CREB-3, cAMP-responsive element-binding protein 3, Leucine zipper protein, Luman, Transcription factor LZIP-alpha, N-terminal Luman, Transcriptionally active form, CREB3, LZIP
Antibody Type
Polyclonal Antibody
Species
Human
Uniprot ID
O43889
Immunogen
Recombinant human Cyclic AMP-responsive element-binding protein 3 protein (1-230AA)
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
ELISA, WB, IHC ;Recommended dilution:WB:1:500-1000, IHC:1:20-200
Background / Function
Endoplasmic reticulum (ER)-bound transcription factor that plays a role in the unfolded protein response (UPR). Involved in cell proliferation and migration, tumor suppression and inflammatory gene expression. Plays also a role in the human immunodeficiency virus type 1 (HIV-1) virus protein expression and in the herpes simplex virus-1 (HSV-1) latent infection and reactivation from latency. Isoform 2 plays a role in the unfolded protein response (UPR). Isoform 2 acts as a positive regulator of LKN-1/CCL15-induced chemotaxis signaling of leukocyte cell migration. Isoform 2 may play a role as a cellular tumor suppressor that is targeted by the hepatitis C virus (HSV) core protein. Isoform 2 represses the VP16-mediated transactivation of immediate early genes of the HSV-1 virus by sequestring host cell factor-1 HCFC1 in the ER membrane of sensory neurons, thereby preventing the initiation of the replicative cascade leading to latent infection. Isoform 3 functions as a negative transcriptional regulator in ligand-induced transcriptional activation of the glucocorticoid receptor NR3C1 by recruiting and activating histone deacetylases (HDAC1, HDAC2 and HDAC6). Isoform 3 decreases the acetylation level of histone H4. Isoform 3 does not promote the chemotactic activity of leukocyte cells. Processed cyclic AMP-responsive element-binding protein 3: acts as a transcription factor that activates unfolded protein response (UPR) target genes during endoplasmic reticulum (ER) stress response. Promotes cell survival against ER stress-induced apoptotic cell death during UPR. Activates transcription from CRE and C/EBP-containing reporter genes. Induces transcriptional activation of chemokine receptors. Activates transcription of genes required for reactivation of the latent HSV-1 virus. Down-regulates Tat-dependent transcription of the HIV-1 LTR by interacting with HIV-1 Tat. It’s transcriptional activity is inhibited by CREBZF in a HCFC1-dependent manner, by the viral transactivator protein VP16 and by the HCV core protein. Binds DNA to the cAMP response element (CRE) (consensus: 5′-GTGACGT[AG][AG]-3′) and C/EBP sequences present in many viral and cellular promoters. Binds to the unfolded protein respons element (UPRE) consensus sequences sites. Binds DNA to the 5′-CCAC[GA]-3’half of ERSE II (5′-ATTGG-N-CCACG-3′). Associates with chromatin to the HERPUD1 promoter.
Isotype
IgG
Conjugate
Unconjugated
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antigen Affinity purified
Literature
[1]DC-STAMP interacts with ER-resident transcription factor LUMAN which becomes activated during DC maturation.”Eleveld-Trancikova D., Sanecka A., van Hout-Kuijer M.A., Looman M.W., Hendriks I.A., Jansen B.J., Adema G.J.Mol. Immunol. 47:1963-1973(2010). [2]A novel protein, Luman/CREB3 recruitment factor, inhibits Luman activation of the unfolded protein response.”Audas T.E., Li Y., Liang G., Lu R.Mol. Cell. Biol. 28:3952-3966(2008).Additional information
Size | 50μl, 100μl |
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