NCBI Protein: NP _004370.1
Peptide sequence around phosphorylation site of serine 129 (I-L-S(p)-R-R) derived from Human CREB.
Human Mouse Rat
WB IHC Recommended dilution: Predicted MW: 43kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,
Background / Function
This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.Chrystelle V. Garat, et al. (2006) Mol. Cell. Biol ; 26: 4934 – 4948.Lilah Rothem,et al. (2004) Mol. Pharmacol ; 66: 1536 – 1543.Darren R. Tyson, et,al. (2002) Endocrinology; 143: 674.Kyung-Woo Park, et al. (2003) Arterioscler. Thromb. Vasc. Biol ; 23: 1364.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.