CREB(Phospho-Ser133) Antibody

$476.70

Cat. No.: 38745 Categories: ,

Description

Aliases

CREB-1; CREB1;

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: P16220
NCBI Protein: NP _004370.1

Immunogen

Peptide sequence around phosphorylation site of serine 133 (R-P-S(p)-Y-R) derived from Human CREB.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IHC Recommended dilution: Predicted MW: 43kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Background / Function

CREB1 is a transcription factor that is a member of the bZIP family of DNA binding proteins. It binds as a homodimer to the CRE (cAMP-Responsive Element), an octameric palindrome containing a conserved core sequence, 5-prime-TGACG-3-prime. It consists of two transcript variants encoding respective isoforms produced by alternate splicing. It is mapped to 2q32.3-q34 . It is phosphorylated by several protein kinases and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. CREB1 is crucial for the consolidation of long-term conditioned fear memories, but not for encoding, storage, or retrieval of these memories. It is required for the stability of reactivated or retrieved conditioned fear memories . Xing J, et al. (1998) Mol Cell Biol 18(4): 1946-55. Tan Y, et al. (1996) EMBO J; 15(17): 4629-42. Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385. Mayo LD, et al. (2001) Biol Chem; 276(27): 25184-9.

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Ser133

Additional information

Size

100?l

Certificate of Analysis