Cyclin D2 (Phospho-Thr280) Antibody

$454.00

Cat. No.: 38687 Categories: ,

Description

Aliases

cyclin D2; G1/S-specific cyclin D2

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot#:P30279;
NCBI Gene#:894

Immunogen

Peptide sequence around phosphorylation site of threonine 280 (A-S-T(p)-P-T) derived from Human Cyclin D2.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB Recommended dilution: Western blotting: 1:500~1:3000,

Background / Function

Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex By similarity.

Conjugate

Unconjugated

Storage Buffer

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Thr280

Additional information

Size

100?l

Certificate of Analysis