def Antibody, FITC conjugated

$210.00$377.00

Cat. No.: 43792 Categories: ,

Description

Aliases

PDF, Polypeptide deformylase, b3287, JW3248, fms, def

Antibody Type

Polyclonal Antibody

Species

Escherichia coli

Uniprot ID

P0A6K3

Immunogen

Recombinant Escherichia coli Peptide deformylase protein (2-169AA)

Raised In

Rabbit

Species Reactivity

Escherichia coli

Tested Applications

ELISA;Not yet tested in other applications.

Background / Function

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.

Isotype

IgG

Conjugate

FITC

Storage Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Caprylic Acid Ammonium Sulfate Precipitation purified

Modification

Peptide deformylase protein

Literature

[1]”A new subclass of the zinc metalloproteases superfamily revealed by the solution structure of peptide deformylase.” Meinnel T., Blanquet S., Dardel F. J. Mol. Biol. 262:375-386(1996) . [2]”Isolation and crystallization of functionally competent Escherichia coli peptide deformylase forms containing either iron or nickel in the active site.” Groche D., Becker A., Schlichting I., Kabsch W., Schultz S., Wagner A.F. Biochem. Biophys. Res. Commun. 246:342-346(1998). [3]”The Escherichia coli fmt gene, encoding methionyl-tRNA(fMet) formyltransferase, escapes metabolic control.” Meinnel T., Guillon J.-M., Mechulam Y., Blanquet S. J. Bacteriol. 175:993-1000(1993).

Additional information

Size

50μg, 100μg

Certificate of Analysis