Aliases
PDF, Polypeptide deformylase, b3287, JW3248, fms, def
Antibody Type
Polyclonal Antibody
Species
Escherichia coli
Uniprot ID
P0A6K3
Immunogen
Recombinant Escherichia coli Peptide deformylase protein (2-169AA)
Raised In
Rabbit
Species Reactivity
Escherichia coli
Tested Applications
ELISA;Not yet tested in other applications.
Background / Function
Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.
Isotype
IgG
Conjugate
FITC
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Modification
Peptide deformylase protein
Literature
[1]”A new subclass of the zinc metalloproteases superfamily revealed by the solution structure of peptide deformylase.” Meinnel T., Blanquet S., Dardel F. J. Mol. Biol. 262:375-386(1996) . [2]”Isolation and crystallization of functionally competent Escherichia coli peptide deformylase forms containing either iron or nickel in the active site.” Groche D., Becker A., Schlichting I., Kabsch W., Schultz S., Wagner A.F. Biochem. Biophys. Res. Commun. 246:342-346(1998). [3]”The Escherichia coli fmt gene, encoding methionyl-tRNA(fMet) formyltransferase, escapes metabolic control.” Meinnel T., Guillon J.-M., Mechulam Y., Blanquet S. J. Bacteriol. 175:993-1000(1993).
