Description
Aliases
ERBB1; Receptor protein-tyrosine kinase ErbB-1; kinase EGFR
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P00533
NCBI Protein: NP_005219.2
Immunogen
Peptide sequence around phosphorylation site of threonine 678 (K-R-T(p)-L-R) derived from Human EGFR.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC Recommended dilution: Predicted MW: 175kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin. Doherty JK, et al. (1999) Proc Natl Acad Sci U S A; 96(19): 10869-10874 Wu TT, et al. (1998) Mol Biol Cell; 9(7): 1661-1674 O
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Thr678
Additional information
Size | 100?l |
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