EGFR(Phospho-Thr678) Antibody

$454.00

Cat. No.: 38792 Categories: ,

Description

Aliases

ERBB1; Receptor protein-tyrosine kinase ErbB-1; kinase EGFR

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: P00533
NCBI Protein: NP_005219.2

Immunogen

Peptide sequence around phosphorylation site of threonine 678 (K-R-T(p)-L-R) derived from Human EGFR.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IHC Recommended dilution: Predicted MW: 175kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Background / Function

Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin. Doherty JK, et al. (1999) Proc Natl Acad Sci U S A; 96(19): 10869-10874 Wu TT, et al. (1998) Mol Biol Cell; 9(7): 1661-1674 O

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Thr678

Additional information

Size

100?l

Certificate of Analysis