ERBB1; Receptor protein-tyrosine kinase ErbB-1; kinase EGFR
NCBI Protein: NP_005219.2
Peptide sequence around phosphorylation site of tyrosine1092 (P-E-Y(p)-I-N) derived from Human EGFR.
Human Mouse Rat
WB IHC Recommended dilution: Predicted MW: 175kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,
Background / Function
Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.Buerger C, et al. (2003) J Biol Chem; 278(39): 37610-21.Wang XQ, (2003) J Biol Chem; 278(49): 48770-8.Saito T, et al. (2004) Endocrinology; 145(9): 4232-43.Pao W, et al. (2004) Proc Natl Acad Sci U S A; 101(36): 13306-11.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.