eIF4E(Phospho-Ser209) Antibody

$454.00

Cat. No.: 39017 Categories: ,

Description

Aliases

mRNA cap-binding protein; eIF-4F 25 kDa subunit;

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: P06730
NCBI Protein: NP_001124150.

Immunogen

Peptide sequence around phosphorylation site of serine 209 (S-G-S(p)-T-T) derived from Human eIF4E.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IHC IF Recommended dilution: Predicted MW: 25kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Background / Function

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Li BD, et al. (1998) Ann Surg; 227(5): 756-763 Altmann M, et al. (1989) Nucleic Acids Res; 17(18): 7520 De Gregorio E, et al. (2001) RNA; 7(1): 106-113 Gu W, et al. (2004) Nucleic Acids Res; 32(15): 4448-4461

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Ser209

Additional information

Size

100?l

Certificate of Analysis