NCBI Gene ID: 2002
Peptide sequence around phosphorylation site of threonine 417(L-S-T(p)-P-V) derived from Human Elk1.
Human Mouse Rat
IHC IF Recommended dilution: Predicted MW: 47kdImmunohistochemistry: 1:50~1:100Immunofluorescence: 1:100~1:200
Background / Function
Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.Yang S.-H., Sharrocks A.D.EMBO J. 24:2161-2171(2005)Zhang L., Yang S.H., Sharrocks A.D.Mol. Cell. Biol. 27:2861-2869(2007)
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.