N-[ε-Maleimidocaproic acid] hydrazide (EMCH) is a water-soluble, heterobifunctional, membrane permeable crosslinker containing a sulfhydryl reactive maleimide group and carbonyl reactive hydrazide group at each end of a 6-carbon spacer arm. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thiol ether bonds, along with release of the maleimide leaving group. Proteins with cysteine residues not involved in disulfide bond formation are targets for these maleimide reactive groups. Carbonyl groups can be formed by ring sugar reduction to form aldehydes which react with hydrazides at pH range of 5.5-7.5.
Synonyms: E-Maleimidocaproic acid hydrazide hydrochloride, EMCH
Name: E-maleimidocaproic acid hydrazide.HCl
Molecular Weight: 261.8
Color & Form: White to off white solid
Melting Point: 105° C