ER; ESR; ESR1; ESTR; ESTRA
NCBI Protein: NP_000116.2
Peptide sequence around phosphorylation site of serine 104 (S-V-S(p)-P-S) derived from Human Estrogen Receptor-a.
WB IHC Recommended dilution: Predicted MW: 66kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,
Background / Function
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.Dittadi, R. et al. (2000) J. Natl. Cancer Inst. 92, 1443-1444.Muthuswamy, S. K. et al. (1999) Mol. Cell. Biol. 19, 6845-6857.Qian, X. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 1500-1504.Kwon, Y. K. et al. (1997) J. Neurosci. 17, 8293-8299.Klapper, L. N. et al. (2000) Cancer Res. 60, 3384-3388.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.