Aliases
ER; ESR; ESR1; ESTR; ESTRA
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P03372
NCBI Protein: NP_000116.2
Immunogen
Peptide sequence around phosphorylation site of serine 104 (S-V-S(p)-P-S) derived from Human Estrogen Receptor-a.
Raised In
Rabbit
Species Reactivity
Human Mouse
Tested Applications
WB IHC Recommended dilution: Predicted MW: 66kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,
Background / Function
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.Dittadi, R. et al. (2000) J. Natl. Cancer Inst. 92, 1443-1444.Muthuswamy, S. K. et al. (1999) Mol. Cell. Biol. 19, 6845-6857.Qian, X. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 1500-1504.Kwon, Y. K. et al. (1997) J. Neurosci. 17, 8293-8299.Klapper, L. N. et al. (2000) Cancer Res. 60, 3384-3388.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser104
