ER; ESR; ESR1; ESTR; ESTRA
NCBI Protein: NP_000116.2
Peptide sequence around phosphorylation site of serine106 (S-P- S(p)-P-L) derived from Human Estrogen Receptor-a.
WB IHC IF Recommended dilution: Predicted MW: 66kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.Marone R, et al. (2004) Nat Cell Biol; 6(6): 515-22.Ren Z, et al. (2002) J Biol Chem; 277(41): 38486-93.Ouyang X,et al. (1998)Carcinogenesis; 19(11): 2013-9.Ouyang X,et al. (1998) Exp Cell Res; 241(2): 467-75.Klapper, L. N. et al. (2000) Cancer Res. 60, 3384-3388.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.