Recombinant human Eyes absent homolog 2 protein (1-514AA)
ELISA, IHC;Recommended dilution:IHC:1:20-1:200
Background / Function
Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5. Tyrosine phosphatase that dephosphorylates ‘Tyr-142’ of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. ‘Tyr-142’ phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis. Plays an important role in hypaxial muscle development together with SIX1 and DACH2; in this it is functionally redundant with EYA1.
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Caprylic Acid Ammonium Sulfate Precipitation purified
Epigenetics and Nuclear Signaling
Literature”Structure-function analyses of the human SIX1-EYA2 complex reveal insights into metastasis and BOR syndrome.” Patrick A.N., Cabrera J.H., Smith A.L., Chen X.S., Ford H.L., Zhao R.Nat. Struct. Mol. Biol. 20:447-453(2013) .