Ezrin(Phospho-Tyr353) Antibody

$454.00

Cat. No.: 38748 Categories: ,

Description

Aliases

Cytovillin; EZRI; VIL2; Villin 2; p81

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: P15311
NCBI Protein: NP_001104547.

Immunogen

Peptide sequence around phosphorylation site of tyrosine 353 (Q-D-Y(p)-E-E) derived from Human EZRIN.

Raised In

Rabbit

Species Reactivity

Human Mouse

Tested Applications

WB IHC Recommended dilution: Predicted MW: 81kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Background / Function

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. Zhao H, et al. (2004) Proc Natl Acad Sci U S A101 (25): 9485-9490. Wang Q, et al. (2003) J Biol Chem 278(48): 47731-47743. Gautreau A, et al. (1999) Proc Natl Acad Sci U S A 96(13): 7300-7305. Crepaldi T, et al. (1997) J Cell Biol 138(2): 423-34.

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Tyr353

Additional information

Size

100?l

Certificate of Analysis