Cytovillin; EZRI; VIL2; Villin 2; p81
NCBI Protein: NP_001104547.
Peptide sequence around phosphorylation site of tyrosine 353 (Q-D-Y(p)-E-E) derived from Human EZRIN.
WB IHC Recommended dilution: Predicted MW: 81kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. Zhao H, et al. (2004) Proc Natl Acad Sci U S A101 (25): 9485-9490. Wang Q, et al. (2003) J Biol Chem 278(48): 47731-47743. Gautreau A, et al. (1999) Proc Natl Acad Sci U S A 96(13): 7300-7305. Crepaldi T, et al. (1997) J Cell Biol 138(2): 423-34.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.