Description
Aliases
FADK 1; FAK1; PTK2
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q05397
NCBI Protein: NP_005598.3
Immunogen
Peptide sequence around phosphorylation site of tyrosine 576/tyrosine 577 (S-T-Y(p)-Y(p)-K-A) derived from Human FAK.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IF Recommended dilution: Predicted MW: 125kd, Western blotting: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background / Function
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity. Parsons, J.T. et al. (2000) Oncogene 19, 5606-5613 Schaller, M.D. et al. (1994) Mol. Cell. Biol. 14, 1680-1688. Cobb, B.S. et al. (1994) Mol. Cell. Biol. 14, 147-155. Chen, H.C. et al. (1996) J. Biol. Chem. 271, 26329-26334.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Tyr576/Tyr577
Additional information
| Size | 100?l |
|---|
