FADK 1; FAK1; PTK2
NCBI Protein: NP_005598.3
Peptide sequence around phosphorylation site of tyrosine 925 (K-V-Y(p)-E-N) derived from Human FAK.
Human Mouse Rat
WB IF Recommended dilution: Predicted MW: 125kd, Western blotting: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background / Function
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.Sanders MA, et al. (2005) J Biol Chem; 280(25): 23516-22.Cherubini A, et al. (2005) Mol Biol Cell; 16(6): 2972-83.Toriumi Y, et al. (2003) FEBS Lett; 553(3): 419-22.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.