FAK(Phospho-Tyr925) Antibody

$476.70

Cat. No.: 38773 Categories: ,

Description

Aliases

FADK 1; FAK1; PTK2

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: Q05397
NCBI Protein: NP_005598.3

Immunogen

Peptide sequence around phosphorylation site of tyrosine 925 (K-V-Y(p)-E-N) derived from Human FAK.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IF Recommended dilution: Predicted MW: 125kd, Western blotting: 1:500~1:1000, Immunofluorescence: 1:100~1:200

Background / Function

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.Sanders MA, et al. (2005) J Biol Chem; 280(25): 23516-22.Cherubini A, et al. (2005) Mol Biol Cell; 16(6): 2972-83.Toriumi Y, et al. (2003) FEBS Lett; 553(3): 419-22.

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Tyr925

Additional information

Size

100?l

Certificate of Analysis