FAN1 Antibody, FITC conjugated

Conjugate: FITC. Product Type: Polyclonal Antibody. Clonality: Polyclonal. Isotype: IgG. Form: Liquid. Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Raised in: Rabbit. Aliases: 6030441H18Rik antibody; Coiled coil domain containing protein MTMR15 antibody; DKFZp451H236 antibody; DKFZp686K16147 antibody; FAN1 antibody; FAN1_HUMAN antibody; FANCD2 and FANCI associated nuclease 1 antibody; FANCD2/FANCI associated nuclease 1 antibody; FANCD2/FANCI-associated nuclease 1 antibody; Fanconi associated nuclease 1 antibody; Fanconi-associated nuclease 1 antibody; hFAN1 antibody; KIAA1018 antibody; KMIN antibody; MTMR 15 antibody; MTMR15 antibody; Myotubularin related protein 15 antibody; Myotubularin-related protein 15 antibody. Immunogen Species: Homo sapiens (Human). UniProt ID: Q9Y2M0. Immunogen: Recombinant Human Fanconi-associated nuclease 1 protein (167-373AA). Species Reactivity: Human. Tested Applications: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500. Background: Nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates (PubMed:20603015, PubMed:20603016, PubMed:20603073, PubMed:20671156, PubMed:24981866, PubMed:25430771). Not involved in DNA double-strand breaks resection (PubMed:20603015, PubMed:20603016). Acts as a 5\’-3\’ exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions. Probably keeps excising with 3\’-flap annealing until it reaches and unhooks the ICL (PubMed:25430771). Acts at sites that have a 5\’-terminal phosphate anchor at a nick or a 1- or 2-nucleotide flap and is augmented by a 3\’ flap (PubMed:25430771). Also has endonuclease activity toward 5\’-flaps (PubMed:20603015, PubMed:20603016, PubMed:24981866). Purification Method: >95%, Protein G purified. Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4. Target Names: FAN1.