Aliases
FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: O43524
NCBI Gene ID: 2309
NCBI
Immunogen
Peptide sequence around phosphorylation site of serine 253(A-V-S(p)-M-D) derived from Human FKHRL1.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC IF Recommended dilution: Predicted MW: 97kdWestern blotting: 1:500~1:1000Immunohistochemistry: 1:50~1:100Immunofluorescence: 1:100~1:200
Background / Function
Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5′-[AG]TAAA[TC]A-3′. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3’UTR of MYC transcript and prevent its translation.Lehtinen M.K., Yuan Z., Boag P.R., Yang Y., Villen J., Becker E.B.E., DiBacco S., de la Iglesia N., Gygi S.P., Blackwell T.K., Bonni A.Cell 125:987-1001(2006) Morishita D., Katayama R., Sekimizu K., Tsuruo T., Fujita N.Cancer Res. 68:5076-5085(2008)Kress T.R., Cannell I.G., Brenkman A.B., Samans B., Gaestel M., Roepman P., Burgering B.M., Bushell M., Rosenwald A., Eilers M.Mol. Cell 41:445-457(2011)
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser253
