FKHRL1(Phospho-Ser253) Antibody

$476.70

Cat. No.: 39065 Categories: ,

Description

Aliases

FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: O43524
NCBI Gene ID: 2309
NCBI

Immunogen

Peptide sequence around phosphorylation site of serine 253(A-V-S(p)-M-D) derived from Human FKHRL1.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IHC IF Recommended dilution: Predicted MW: 97kdWestern blotting: 1:500~1:1000Immunohistochemistry: 1:50~1:100Immunofluorescence: 1:100~1:200

Background / Function

Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5′-[AG]TAAA[TC]A-3′. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3’UTR of MYC transcript and prevent its translation.Lehtinen M.K., Yuan Z., Boag P.R., Yang Y., Villen J., Becker E.B.E., DiBacco S., de la Iglesia N., Gygi S.P., Blackwell T.K., Bonni A.Cell 125:987-1001(2006) Morishita D., Katayama R., Sekimizu K., Tsuruo T., Fujita N.Cancer Res. 68:5076-5085(2008)Kress T.R., Cannell I.G., Brenkman A.B., Samans B., Gaestel M., Roepman P., Burgering B.M., Bushell M., Rosenwald A., Eilers M.Mol. Cell 41:445-457(2011)

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Ser253

Additional information

Size

100?l

Certificate of Analysis