Description
Aliases
Fzr, CDC20-like protein 1, hCDH1, Cdh1/Hct1 homolog, CDH1, FYR, FZR, KIAA1242, FZR1
Antibody Type
Polyclonal Antibody
Species
Human
Uniprot ID
Q9UM11
Immunogen
Recombinant human Fizzy-related protein (1-493AA)
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
ELISA;Not yet tested in other applications.
Background / Function
Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.
Isotype
IgG
Conjugate
HRP
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Modification
Fizzy-related protein homolog
Literature
[1]”Male germ cell-associated kinase is overexpressed in prostate cancer cells and causes mitotic defects via deregulation of APC/C(CDH1).” Wang L.Y., Kung H.J. Oncogene 31:2907-2918(2011). [2]”SIRT2 maintains genome integrity and suppresses tumorigenesis through regulating APC/C activity.” Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer Cell 20:487-499(2010). [3]”Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.” Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2009).Additional information
| Size | 50μg, 100μg |
|---|
