Description
Aliases
G3BP; GAP SH3-domain binding protein 1;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q13283
NCBI Protein: NP_005745.1
Immunogen
Peptide sequence around phosphorylation site of serine 232 (S-S-S(p)-P-A) derived from Human G3BP-1.
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
WB IHC IF Recommended dilution: Predicted MW: 60kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3′-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3′ tail or hanging tails at both 5′- and 3′-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5′ to 3′ direction along the bound single-stranded DNA.Tao WA, et al. (2005) Nat Methods. 2(8): 591-598.Zheng H, et al. (2005) Mol Cell Proteomics. 4(6):721-730.Beausoleil SA, et al. (2004) Proc Natl Acad Sci U S A.101 (33): 12130-12135.Tourrihre H, et al. (2003) J Cell Biol.160 (6): 823-831.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser232
Additional information
| Size | 100?l |
|---|
